Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of  a Family 5 Glycoside Hydrolase

Roman Brunecky; Bryon Donohoe; Hui Wei; Ashutosh Mittal; Michael Himmel; Vladimir Lunin; Todd Shollenberger

doi:10.1038/s41598-017-04502-1

Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

Roman Brunecky
, Bryon Donohoe
, Hui Wei
, Ashutosh Mittal
, Michael Himmel
, Vladimir Lunin
, Todd Shollenberger

Research output: Contribution to journal › Article › peer-review

12 Scopus Citations

Abstract

In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1-4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.

Original language	American English
Article number	Article No. 4389
Number of pages	9
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-04502-1 https://doi.org/10.1038/s41598-017-04502-1
State	Published - 2017

Bibliographical note

Publisher Copyright:
© 2017 The Author(s).

NLR Publication Number

NREL/JA-2700-67215

Keywords

AcCel5A endoglucanase
biomass
in planta expression
plant cell wall
recalcitrance

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Cite this

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title = "Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase",

abstract = "In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1-4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.",

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AU - Brunecky, Roman

AU - Donohoe, Bryon

AU - Wei, Hui

AU - Mittal, Ashutosh

AU - Himmel, Michael

AU - Lunin, Vladimir

AU - Shollenberger, Todd

PY - 2017

Y1 - 2017

N2 - In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1-4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.

AB - In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1-4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.

KW - AcCel5A endoglucanase

KW - biomass

KW - in planta expression

KW - plant cell wall

KW - recalcitrance

UR - https://www.scopus.com/pages/publications/85021657550

U2 - 10.1038/s41598-017-04502-1

DO - 10.1038/s41598-017-04502-1

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JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - Article No. 4389

ER -