Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

Stefanie Van Wychen; Lieve Laurens

doi:10.1007/978-1-0716-5031-8_10

Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or liquid chromatography, specifically high-performance anion exchange method, for a more specific monomeric sugar profile.

Original language	American English
Title of host publication	Biofuels from Algae: Methods and Protocols, Second Edition
Subtitle of host publication	Methods in Molecular Biology, vol 2996
Editors	K. Spilling
Pages	101-112
DOIs	https://doi.org/10.1007/978-1-0716-5031-8_10
State	Published - 2026

Bibliographical note

For first edition, see NREL/CH-2700-70676

NLR Publication Number

NLR/CH-2700-100684

Keywords

carbohydrate
liquid chromatography
spectrophotometry

Access to Document

10.1007/978-1-0716-5031-8_10

Cite this

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title = "Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography",

abstract = "The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or liquid chromatography, specifically high-performance anion exchange method, for a more specific monomeric sugar profile.",

keywords = "carbohydrate, liquid chromatography, spectrophotometry",

author = "\{Van Wychen\}, Stefanie and Lieve Laurens",

note = "For first edition, see NREL/CH-2700-70676",

year = "2026",

doi = "10.1007/978-1-0716-5031-8\_10",

language = "American English",

pages = "101--112",

editor = "K. Spilling",

booktitle = "Biofuels from Algae: Methods and Protocols, Second Edition",

}

Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography. / Van Wychen, Stefanie ; Laurens, Lieve.
Biofuels from Algae: Methods and Protocols, Second Edition: Methods in Molecular Biology, vol 2996. ed. / K. Spilling. 2026. p. 101-112.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

AU - Van Wychen, Stefanie

AU - Laurens, Lieve

N1 - For first edition, see NREL/CH-2700-70676

PY - 2026

Y1 - 2026

N2 - The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or liquid chromatography, specifically high-performance anion exchange method, for a more specific monomeric sugar profile.

AB - The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or liquid chromatography, specifically high-performance anion exchange method, for a more specific monomeric sugar profile.

KW - carbohydrate

KW - liquid chromatography

KW - spectrophotometry

U2 - 10.1007/978-1-0716-5031-8_10

DO - 10.1007/978-1-0716-5031-8_10

M3 - Chapter

SP - 101

EP - 112

BT - Biofuels from Algae: Methods and Protocols, Second Edition

A2 - Spilling, K.

ER -

Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography

Abstract

Bibliographical note

NLR Publication Number

Keywords

Access to Document

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Cite this