TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate

Gregg Beckham; Michelle Reed; Jason DesVeaux; Brenna Norton-Baker; Natasha Murphy

doi:10.2172/3018255

TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate

National Laboratory of the Rockies
National Renewable Energy Laboratory

Research output: NLR › Technical Report

Abstract

The primary objective of this project was to develop a new polyethylene terephthalate (PET) hydrolase enzyme to depolymerize relevant PET substrates for Birch Biosciences, using high-throughput protein expression, purification, and assaying systems and machine learning-guided enzyme design. As a secondary project objective, we also aimed to develop a more energy-efficient ethylene glycol (EG) recovery strategy relative to distillation.

Original language	American English
Number of pages	9
DOIs	https://doi.org/10.2172/3018255
State	Published - 2026

NLR Publication Number

NLR/TP-2A00-98395

Keywords

crystalline PET
Enzymes
PET hydrolase

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10.2172/3018255

https://www.nlr.gov/docs/fy26osti/98395.pdf

Cite this

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title = "TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate",

abstract = "The primary objective of this project was to develop a new polyethylene terephthalate (PET) hydrolase enzyme to depolymerize relevant PET substrates for Birch Biosciences, using high-throughput protein expression, purification, and assaying systems and machine learning-guided enzyme design. As a secondary project objective, we also aimed to develop a more energy-efficient ethylene glycol (EG) recovery strategy relative to distillation.",

keywords = "crystalline PET, Enzymes, PET hydrolase",

author = "Gregg Beckham and Michelle Reed and Jason DesVeaux and Brenna Norton-Baker and Natasha Murphy",

year = "2026",

doi = "10.2172/3018255",

language = "American English",

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T1 - TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate

AU - Beckham, Gregg

AU - Reed, Michelle

AU - DesVeaux, Jason

AU - Norton-Baker, Brenna

AU - Murphy, Natasha

PY - 2026

Y1 - 2026

N2 - The primary objective of this project was to develop a new polyethylene terephthalate (PET) hydrolase enzyme to depolymerize relevant PET substrates for Birch Biosciences, using high-throughput protein expression, purification, and assaying systems and machine learning-guided enzyme design. As a secondary project objective, we also aimed to develop a more energy-efficient ethylene glycol (EG) recovery strategy relative to distillation.

AB - The primary objective of this project was to develop a new polyethylene terephthalate (PET) hydrolase enzyme to depolymerize relevant PET substrates for Birch Biosciences, using high-throughput protein expression, purification, and assaying systems and machine learning-guided enzyme design. As a secondary project objective, we also aimed to develop a more energy-efficient ethylene glycol (EG) recovery strategy relative to distillation.

KW - crystalline PET

KW - Enzymes

KW - PET hydrolase

U2 - 10.2172/3018255

DO - 10.2172/3018255

M3 - Technical Report

ER -

TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate

Abstract

NLR Publication Number

Keywords

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Cite this