Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction

Seon Ah Kim; Gregg Beckham; Mikael Gudmundsson; Miao Wu; Takuya Ishida; Majid Momeni; Gustav Vaaje-Kolstad; Daniel Lundberg; Antoine Royant; Jerry Ståhlberg; Vincent Eijsink; Mats Sandgren

doi:10.1074/jbc.M114.563494

Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction

Seon Ah Kim
, Gregg Beckham
, Mikael Gudmundsson
, Miao Wu
, Takuya Ishida
, Majid Momeni
, Gustav Vaaje-Kolstad
, Daniel Lundberg
, Antoine Royant
, Jerry Ståhlberg
, Vincent Eijsink
, Mats Sandgren

Swedish University of Agricultural Sciences
The University of Tokyo
Norwegian University of Life Sciences
European Synchrotron Radiation Facility
Grenoble Alpes University

Research output: Contribution to journal › Article › peer-review

113 Scopus Citations

Abstract

Background: Lytic polysaccharide monooxygenases (LPMOs) exhibit a copper center that binds dioxygen for catalysis. Results: We present LPMO structures from Cu(II) to Cu(I) and analyze the transition with quantum mechanical calculations. Conclusion: Reduction changes the copper coordination state but requires only minor structural and electronic changes. Significance: These structures provide insight into LPMO catalytic activation for further mechanistic studies.

Original language	American English
Pages (from-to)	18782-18792
Number of pages	11
Journal	Journal of Biological Chemistry
Volume	289
Issue number	27
DOIs	https://doi.org/10.1074/jbc.M114.563494 https://doi.org/10.1074/jbc.M114.563494
State	Published - 4 Jul 2014

NLR Publication Number

NREL/JA-5100-62549

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Kim, S. A., Beckham, G., Gudmundsson, M., Wu, M., Ishida, T., Momeni, M., Vaaje-Kolstad, G., Lundberg, D., Royant, A., Ståhlberg, J., Eijsink, V., & Sandgren, M. (2014). Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction. Journal of Biological Chemistry, 289(27), 18782-18792. https://doi.org/10.1074/jbc.M114.563494, https://doi.org/10.1074/jbc.M114.563494

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title = "Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction",

abstract = "Background: Lytic polysaccharide monooxygenases (LPMOs) exhibit a copper center that binds dioxygen for catalysis. Results: We present LPMO structures from Cu(II) to Cu(I) and analyze the transition with quantum mechanical calculations. Conclusion: Reduction changes the copper coordination state but requires only minor structural and electronic changes. Significance: These structures provide insight into LPMO catalytic activation for further mechanistic studies.",

author = "Kim, \{Seon Ah\} and Gregg Beckham and Mikael Gudmundsson and Miao Wu and Takuya Ishida and Majid Momeni and Gustav Vaaje-Kolstad and Daniel Lundberg and Antoine Royant and Jerry St{\aa}hlberg and Vincent Eijsink and Mats Sandgren",

year = "2014",

month = jul,

day = "4",

doi = "10.1074/jbc.M114.563494",

language = "American English",

volume = "289",

pages = "18782--18792",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

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Kim, SA , Beckham, G, Gudmundsson, M, Wu, M, Ishida, T, Momeni, M, Vaaje-Kolstad, G, Lundberg, D, Royant, A, Ståhlberg, J, Eijsink, V & Sandgren, M 2014, 'Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction', Journal of Biological Chemistry, vol. 289, no. 27, pp. 18782-18792. https://doi.org/10.1074/jbc.M114.563494, https://doi.org/10.1074/jbc.M114.563494

TY - JOUR

T1 - Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction

AU - Kim, Seon Ah

AU - Beckham, Gregg

AU - Gudmundsson, Mikael

AU - Wu, Miao

AU - Ishida, Takuya

AU - Momeni, Majid

AU - Vaaje-Kolstad, Gustav

AU - Lundberg, Daniel

AU - Royant, Antoine

AU - Ståhlberg, Jerry

AU - Eijsink, Vincent

AU - Sandgren, Mats

PY - 2014/7/4

Y1 - 2014/7/4

N2 - Background: Lytic polysaccharide monooxygenases (LPMOs) exhibit a copper center that binds dioxygen for catalysis. Results: We present LPMO structures from Cu(II) to Cu(I) and analyze the transition with quantum mechanical calculations. Conclusion: Reduction changes the copper coordination state but requires only minor structural and electronic changes. Significance: These structures provide insight into LPMO catalytic activation for further mechanistic studies.

AB - Background: Lytic polysaccharide monooxygenases (LPMOs) exhibit a copper center that binds dioxygen for catalysis. Results: We present LPMO structures from Cu(II) to Cu(I) and analyze the transition with quantum mechanical calculations. Conclusion: Reduction changes the copper coordination state but requires only minor structural and electronic changes. Significance: These structures provide insight into LPMO catalytic activation for further mechanistic studies.

UR - https://www.scopus.com/pages/publications/84903852138

U2 - 10.1074/jbc.M114.563494

DO - 10.1074/jbc.M114.563494

M3 - Article

C2 - 24828494

AN - SCOPUS:84903852138

SN - 0021-9258

VL - 289

SP - 18782

EP - 18792

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 27

ER -

Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photoreduction

Abstract

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