Enzyme Scaffolds and Methods of Use

Yannick Bomble; Michael Himmel; Jeffrey Linger; Roman Brunecky; John Aikens

Enzyme Scaffolds and Methods of Use

Yannick Bomble (Inventor)
, Michael Himmel (Inventor)
, Jeffrey Linger (Inventor)
, Roman Brunecky (Inventor)
, John Aikens (Inventor)

Aikens Associates

Research output: Patent

Abstract

Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays

Original language	American English
Patent number	10,577,633 B2
Filing date	3/03/20
State	Published - 2020

NLR Publication Number

NREL/PT-2700-76406

Keywords

dockerins
enzymatic proteins
heterologous enzymes
microorganismal cellulosomes
ohesins
polypeptide scaffolds

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title = "Enzyme Scaffolds and Methods of Use",

abstract = "Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays",

keywords = "dockerins, enzymatic proteins, heterologous enzymes, microorganismal cellulosomes, ohesins, polypeptide scaffolds",

author = "Yannick Bomble and Michael Himmel and Jeffrey Linger and Roman Brunecky and John Aikens",

year = "2020",

language = "American English",

publisher = "National Laboratory of the Rockies (NLR)",

address = "United States",

type = "Patent",

note = "10,577,633 B2",

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TY - PAT

T1 - Enzyme Scaffolds and Methods of Use

AU - Bomble, Yannick

AU - Himmel, Michael

AU - Linger, Jeffrey

AU - Brunecky, Roman

AU - Aikens, John

PY - 2020

Y1 - 2020

N2 - Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays

AB - Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays

KW - dockerins

KW - enzymatic proteins

KW - heterologous enzymes

KW - microorganismal cellulosomes

KW - ohesins

KW - polypeptide scaffolds

M3 - Patent

M1 - 10,577,633 B2

Y2 - 2020/03/03

PB - National Laboratory of the Rockies (NLR)

ER -

Enzyme Scaffolds and Methods of Use

Abstract

NLR Publication Number

Keywords

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Cite this