Abstract
The plasmid used to delete hexR, pGB022, in our Pseudomonas putida KT2440 strain engineered to produce cis,cis-muconic acid from glucose, GB062, inadvertently introduced two mutations: a point mutation in the zwf-1, encoding the glucose-6-phosphate 1-dehydrogenase, that results in a conservative, V157I replacement and a deletion of the last 8 bp of the glucose-6-phosphate 1-epimerase gene, yeaD, leading to a frame shift in this gene and, consequently, the replacement of the last two amino acids of the C-terminus (LS) with 56 amino acids (RGPWVCPWVGKTAGCVSSRLFARSSIVLSVRLTYHLSVTLVCNVVVFTTLSLENRV).
| Original language | American English |
|---|---|
| Pages (from-to) | 66-67 |
| Number of pages | 2 |
| Journal | Metabolic Engineering |
| Volume | 72 |
| DOIs | |
| State | Published - Jul 2022 |
Bibliographical note
See NREL/JA-2A00-75899 for original articleNLR Publication Number
- NREL/JA-2A00-82313
Keywords
- glucose metabolism
- Pseudomonas putida
Fingerprint
Dive into the research topics of 'Corrigendum to "Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440" [Metab. Eng. 59 (2020) 64-75]'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver