Chapter 12: Gene Editing Technologies for Biofuel Production in Thermophilic Microbes

Sharon Smolinski; Emily Freed; Carrie Eckert

doi:10.1007/978-1-0716-0195-2_12

Chapter 12: Gene Editing Technologies for Biofuel Production in Thermophilic Microbes

Sharon Smolinski
, Emily Freed
, Carrie Eckert

Biosciences Center

National Renewable Energy Laboratory
University of Colorado Boulder

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus Citations

Abstract

Thermophilic microbes are an attractive bioproduction platform due to their inherently lower contamination risk and their ability to perform thermostable enzymatic processes which may be required for biomass processing and other industrial applications. The engineering of microbes for industrial scale processes requires a suite of genetic engineering tools to optimize existing biological systems as well as to design and incorporate new metabolic pathways within strains. Yet, such tools are often lacking and/or inadequate for novel microbes, especially thermophiles. This chapter focuses on genetic tool development and engineering strategies, in addition to challenges, for thermophilic microbes. We provide detailed instructions and techniques for tool development for an anaerobic thermophile, Caldanaerobacter subterraneus subsp. tengcongensis, including culturing, plasmid construction, transformation, and selection. This establishes a foundation for advanced genetic tool development necessary for the metabolic engineering of this microbe and potentially other thermophilic organisms.

Original language	American English
Title of host publication	Metabolic Pathway Engineering
Subtitle of host publication	Methods in Molecular Biology, Volume 2096
Editors	M. E. Himmel, Y. J. Bomble
Publisher	Humana Press Inc.
Pages	149-163
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-0716-0195-2_12 https://doi.org/10.1007/978-1-0716-0195-2_12
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2096
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

NLR Publication Number

NREL/CH-2700-77588

Keywords

Biofuel
Caldanaerobacter subterraneus subsp. tengcongensis
Genetic engineering
Genetic tools
Thermophile
Transformation

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Cite this

Smolinski, S., Freed, E., & Eckert, C. (2020). Chapter 12: Gene Editing Technologies for Biofuel Production in Thermophilic Microbes. In M. E. Himmel, & Y. J. Bomble (Eds.), Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096 (pp. 149-163). (Methods in Molecular Biology; Vol. 2096). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0195-2_12, https://doi.org/10.1007/978-1-0716-0195-2_12

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Smolinski, S, Freed, E & Eckert, C 2020, Chapter 12: Gene Editing Technologies for Biofuel Production in Thermophilic Microbes. in ME Himmel & YJ Bomble (eds), Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096. Methods in Molecular Biology, vol. 2096, Humana Press Inc., pp. 149-163. https://doi.org/10.1007/978-1-0716-0195-2_12, https://doi.org/10.1007/978-1-0716-0195-2_12

Chapter 12: Gene Editing Technologies for Biofuel Production in Thermophilic Microbes. / Smolinski, Sharon; Freed, Emily; Eckert, Carrie.
Metabolic Pathway Engineering: Methods in Molecular Biology, Volume 2096. ed. / M. E. Himmel; Y. J. Bomble. Humana Press Inc., 2020. p. 149-163 (Methods in Molecular Biology; Vol. 2096).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - Chapter 12: Gene Editing Technologies for Biofuel Production in Thermophilic Microbes

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AU - Freed, Emily

AU - Eckert, Carrie

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2020.

PY - 2020

Y1 - 2020

N2 - Thermophilic microbes are an attractive bioproduction platform due to their inherently lower contamination risk and their ability to perform thermostable enzymatic processes which may be required for biomass processing and other industrial applications. The engineering of microbes for industrial scale processes requires a suite of genetic engineering tools to optimize existing biological systems as well as to design and incorporate new metabolic pathways within strains. Yet, such tools are often lacking and/or inadequate for novel microbes, especially thermophiles. This chapter focuses on genetic tool development and engineering strategies, in addition to challenges, for thermophilic microbes. We provide detailed instructions and techniques for tool development for an anaerobic thermophile, Caldanaerobacter subterraneus subsp. tengcongensis, including culturing, plasmid construction, transformation, and selection. This establishes a foundation for advanced genetic tool development necessary for the metabolic engineering of this microbe and potentially other thermophilic organisms.

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KW - Biofuel

KW - Caldanaerobacter subterraneus subsp. tengcongensis

KW - Genetic engineering

KW - Genetic tools

KW - Thermophile

KW - Transformation

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AN - SCOPUS:85088811004

T3 - Methods in Molecular Biology

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ER -

Chapter 12: Gene Editing Technologies for Biofuel Production in Thermophilic Microbes

Abstract

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Keywords

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