Adsorption of Cellobiohydrolases I onto Lignin Fractions from Dilute Acid Pretreated Broussonetia papyrifera

Robert Sykes; Lan Yao; Haitao Yang; Chang Yoo; Xianzhi Meng; Mi Li; Yunqiao Pu; Arthur Ragauskas

doi:10.1016/j.biortech.2017.08.024

Adsorption of Cellobiohydrolases I onto Lignin Fractions from Dilute Acid Pretreated Broussonetia papyrifera

Robert Sykes
, Lan Yao
, Haitao Yang
, Chang Yoo
, Xianzhi Meng
, Mi Li
, Yunqiao Pu
, Arthur Ragauskas

Hubei University of Technology
South China University of Technology
University of Tennessee, Knoxville
Oak Ridge National Laboratory

Research output: Contribution to journal › Article › peer-review

35 Scopus Citations

Abstract

Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.

Original language	American English
Pages (from-to)	957-962
Number of pages	6
Journal	Bioresource Technology
Volume	244
Issue number	Part 1
DOIs	https://doi.org/10.1016/j.biortech.2017.08.024
State	Published - 2017

NLR Publication Number

NREL/JA-5100-70099

Keywords

adsorption
Broussonetia papyrifera
cellobiohydrolases I
dilute acid
lignin fraction
pretreatment

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10.1016/j.biortech.2017.08.024

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@article{208d1dad607e4a4d997e152fc9601382,

title = "Adsorption of Cellobiohydrolases I onto Lignin Fractions from Dilute Acid Pretreated Broussonetia papyrifera",

abstract = "Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.",

keywords = "adsorption, Broussonetia papyrifera, cellobiohydrolases I, dilute acid, lignin fraction, pretreatment",

author = "Robert Sykes and Lan Yao and Haitao Yang and Chang Yoo and Xianzhi Meng and Mi Li and Yunqiao Pu and Arthur Ragauskas",

year = "2017",

doi = "10.1016/j.biortech.2017.08.024",

language = "American English",

volume = "244",

pages = "957--962",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

number = "Part 1",

}

TY - JOUR

T1 - Adsorption of Cellobiohydrolases I onto Lignin Fractions from Dilute Acid Pretreated Broussonetia papyrifera

AU - Sykes, Robert

AU - Yao, Lan

AU - Yang, Haitao

AU - Yoo, Chang

AU - Meng, Xianzhi

AU - Li, Mi

AU - Pu, Yunqiao

AU - Ragauskas, Arthur

PY - 2017

Y1 - 2017

N2 - Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.

AB - Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.

KW - adsorption

KW - Broussonetia papyrifera

KW - cellobiohydrolases I

KW - dilute acid

KW - lignin fraction

KW - pretreatment

U2 - 10.1016/j.biortech.2017.08.024

DO - 10.1016/j.biortech.2017.08.024

M3 - Article

SN - 0960-8524

VL - 244

SP - 957

EP - 962

JO - Bioresource Technology

JF - Bioresource Technology

IS - Part 1

ER -

Adsorption of Cellobiohydrolases I onto Lignin Fractions from Dilute Acid Pretreated Broussonetia papyrifera

Abstract

NLR Publication Number

Keywords

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